In a blender, combine truffle-infused liquid, calcium lactate, and salt. Blend well until smooth.
In a separate bowl, mix sodium alginate with 1 cup of water using an immersion blender until fully dissolved. Let sit for 15 minutes to remove air bubbles.
Using a pipette or spoon, drop small amounts of the truffle mixture into the alginate bath. Let sit for 1–2 minutes until spheres form.
Remove the spheres with a slotted spoon and transfer to a bowl of cold water to rinse. Drain and set aside.